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This module is designed to educate students on the
development of biotechnology, specifically transgenic
plants. This module can be completed in four hours of class
time. Additional time is required to make observations
during the study. If additional extensions of this topic are
desired, up to two additional hours may be spent using extra
suggested activities.
After completion of this module, the student will be able
to:
1. Describe biotechnology
2. Explain Bt corn
1. Plan the arrival time of the corn seed and ECB egg
masses. The corn seed can be ordered from a seed corn
company in your area.
2. Label eight pots (four pots should receive a Bt
label and four pots should receive a NonBt label). Plant
approximately five Bt corn seeds in each of four pots and
five NonBt corn seeds in each of four pots. When the corn
reaches 2-3 leaf stage, order the ECB egg masses.
3. When ECB egg masses arrive, keep them in high
humidity until ready to use in experiments. If the eggs
become too dry, they will not hatch.
4. Eggs may be kept dormant for one week by storing
them in a plastic bag with a damp paper towel in the
refrigerator. If egg masses arrive on wax paper, scrape the
side of the wax paper that does not have eggs on it with a
knife. The egg masses should pop off the wax paper. Collect
the egg masses, in a plastic bag with a damp paper towel and
place in the refrigerator. The damp paper towel will keep
the humidity high in the plastic bag and the cool
temperatures will slow the development of the eggs.
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